Mouse monoclonal antibody raised against partial recombinant human EPCAM.
Immunogen:
Recombinant protein corresponding to amino acids 77-202 at extracellular domain of human EPCAM.
Host:
Mouse
Theoretical MW (kDa):
40-43
Reactivity:
Human
Form:
Liquid
Purification:
Protein A/G purification
Isotype:
IgG2b, kappa
Recommend Usage:
ELISA (2-4 ug/mL for coating) Flow Cytometry (0.5-1 ug/106 cells) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) Western Blotting (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 10 mM PBS.
Storage Instruction:
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq