LGALS1 monoclonal antibody, clone GAL1/1831
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human LGALS1.
Immunogen
Recombinant protein corresponding to amino acids 12-108 of human LGALS1.
Host
Mouse
Theoretical MW (kDa)
14
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, lambda
Recommend Usage
ELISA (2-4 ug/mL for coating)
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blotting (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: K562 and Lane 3: 293 cell lysates with LGALS1 monoclonal antibody, clone GAL1/1831 (Cat # MAB14837).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate carcinoma with LGALS1 monoclonal antibody, clone GAL1/1831 (Cat # MAB14837).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — LGALS1
Entrez GeneID
3956Protein Accession#
P09382Gene Name
LGALS1
Gene Alias
DKFZp686E23103, GAL1, GBP
Gene Description
lectin, galactoside-binding, soluble, 1
Omim ID
150570Gene Ontology
HyperlinkGene Summary
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq
Other Designations
OTTHUMP00000028982|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|galaptin|galectin 1|galectin-1|lactose-binding lectin 1
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Interactome
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Disease
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Publication Reference
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Molecular cloning, characterization, and expression of a human 14-kDa lectin.
Couraud PO, Casentini-Borocz D, Bringman TS, Griffith J, McGrogan M, Nedwin GE.
The Journal of Biological Chemistry 1989 Jan; 264(2):1310.
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Molecular cloning, characterization, and expression of a human 14-kDa lectin.
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