Mouse monoclonal antibody raised against partial recombinant human ELA3B.
Recombinant protein corresponding to amino acids 82-238 of human ELA3B.
Theoretical MW (kDa):
Human, Mouse, Rat
Protein A/G purification
ELISA (2-4 ug/mL for coating) Flow Cytometry (0.5-1 ug/106 cells) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In 10 mM PBS.
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas (A), rat pancreas (B) and mouse pancreas (C) with ELA3B monoclonal antibody, clone CELA3B/1257 (Cat # MAB14788).
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq
OTTHUMP00000002837,elastase 1,elastase 3B,protease E