KRT8/KRT18 monoclonal antibody, clone B22.1/B23.1
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Specification
Product Description
Mouse monoclonal antibody raised against native human KRT8 and KRT18.
Immunogen
A mixture of cytoskeletal preparation from HeLa and PMC-42 cells.
Host
Mouse
Theoretical MW (kDa)
52.5, 45
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with KRT8/KRT18 monoclonal antibody, clone B22.1/B23.1 (Cat # MAB14667).Immunofluorescence
Flow Cytometry
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Gene Info — KRT8
Entrez GeneID
3856Protein Accession#
P05787; P05783Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
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Gene Info — KRT18
Entrez GeneID
3875Protein Accession#
P05787; P05783Gene Name
KRT18
Gene Alias
CYK18, K18
Gene Description
keratin 18
Gene Ontology
HyperlinkGene Summary
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
cell proliferation-inducing protein 46|cytokeratin 18
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Interactome
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Pathway
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Disease
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Publication Reference
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Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1.
B Angus, S Kiberu, J Purvis, L Wilkinson, C H Horne.
The Journal of Pathology 1988 May; 155:71.
Application:IHC-P, Human, Human cervical stratified squamous epithelium.
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NCL-5D3: a new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue.
Angus B, Purvis J, Stock D, Westley BR, Samson AC, Routledge EG, Carpenter FH, Horne CH.
The Journal of Pathology 1987 Dec; 153(4):377.
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Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1.
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