Macrophage monoclonal antibody, clone LN-5
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with Macrophage monoclonal antibody, clone LN-5 (Cat # MAB14660).
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Specification
Product Description
Mouse monoclonal antibody raised against human macrophage.
Immunogen
Human peripheral blood mononuclear cells.
Host
Mouse
Reactivity
Human
Specificity
This monoclonal antibody stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. LN-5 selectively stains human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors are negative, and only sebocytes from the onset of their differentiation reveal positive cytoplasmic staining. Since there are very few selective and easy-to-use markers of sebaceous glands, LN-5 antibody can offer a simple and relatively specific way to detect human sebocytes from the onset of their.
Form
Liquid
Isotype
IgM, kappa
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-0.2 ug/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with Macrophage monoclonal antibody, clone LN-5 (Cat # MAB14660). -
Publication Reference
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LN-5 antibody against human macrophages cross-reacts with routinely processed human sebaceous glands.
Lis GJ, Litwin JA, Furgał-Borzych A, Pyka-Fościak G.
Folia Histochemica et Cytobiologica 2012 Jul; 50(2):319.
Application：IF, IHC-P, Human, Human lungs, Human sebaceous glands, Human skins.
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Lymphomas of true histiocytic origin. Expression of different phenotypes in so-called true histiocytic lymphoma and malignant histiocytosis.
Hsu SM, Ho YS, Hsu PL.
The American Journal of Pathology 1991 Jun; 138(6):1389.
Application：IHC-P, Human, Fresh tissues from lymph nodes or tumor biopsy specimens.
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LN-5 antibody against human macrophages cross-reacts with routinely processed human sebaceous glands.
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