VIM monoclonal antibody, clone VM1170
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human angiosarcoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human uterus with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human leiomyosarcoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rhabdomyosarcoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human ewing's sarcoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human VIM.
Immunogen
Recombinant protein corresponding to full length human VIM.
Host
Mouse
Theoretical MW (kDa)
57-60
Reactivity
Human
Specificity
This antibody shows no cross-reaction with other closely related intermediate filament proteins such as desmin, keratin, neurofilament, and glial fibrillary acid protein.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blot (0.25-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS.
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human angiosarcoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human uterus with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human leiomyosarcoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human melanoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rhabdomyosarcoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human ewing's sarcoma with VIM monoclonal antibody, clone VM1170 (Cat # MAB14648).Immunofluorescence
Flow Cytometry
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Gene Info — VIM
Entrez GeneID
7431Protein Accession#
P08670Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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Publication Reference
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Monoclonal antibodies specific for vimentin.
Osborn M, Debus E, Weber K.
European Journal of Cell Biology 1984 May; 34(1):137.
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Monoclonal antibodies specific for vimentin.
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