KRT76 monoclonal antibody, clone KRTH/1076
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human skin (B) rat stomach and (C) rat oviduct with KRT76 monoclonal antibody, clone KRTH/1076 (Cat # MAB14645).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human KRT76.
Immunogen
Recombinant protein corresponding to full length human KRT76.
Host
Mouse
Theoretical MW (kDa)
67,64,59,58,56,52
Reactivity
Human, Rat
Specificity
This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8).
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA and 0.05% azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human skin (B) rat stomach and (C) rat oviduct with KRT76 monoclonal antibody, clone KRTH/1076 (Cat # MAB14645).Immunofluorescence
Flow Cytometry
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Gene Info — KRT76
Entrez GeneID
51350Protein Accession#
Q01546Gene Name
KRT76
Gene Alias
HUMCYT2A, KRT2B, KRT2P
Gene Description
keratin 76
Gene Ontology
HyperlinkGene Summary
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq
Other Designations
cytokeratin 2|keratin 2p
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Interactome
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Publication Reference
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Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
J Woodcock-Mitchell, R Eichner, W G Nelson, T T Sun.
The Journal of Cell Biology 1982 Nov; 95(2 Pt 1):580.
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Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies.
S C Tseng, M J Jarvinen, W G Nelson, J W Huang, J Woodcock-Mitchell, T T Sun.
Cell 1982 Sep; 30(2):361.
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Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
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