KRT77 monoclonal antibody, clone KRTL/1077
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human skin (B) rat oviduct and (C) rat stomach with KRT77 monoclonal antibody, clone KRTL/1077 (Cat # MAB14644).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human KRT77.
Immunogen
Recombinant protein corresponding to full length human KRT77.
Host
Mouse
Theoretical MW (kDa)
56.5, 50, 48, 40
Reactivity
Human, Rat
Specificity
This antibody recognizes the 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human skin (B) rat oviduct and (C) rat stomach with KRT77 monoclonal antibody, clone KRTL/1077 (Cat # MAB14644).Immunofluorescence
Flow Cytometry
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Gene Info — KRT77
Entrez GeneID
374454Protein Accession#
Q7Z794Gene Name
KRT77
Gene Alias
K1B, KRT1B, MGC148087
Gene Description
keratin 77
Omim ID
611158Gene Ontology
HyperlinkGene Summary
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes an epithelial keratin that is expressed in the skin and eccrine sweat glands. The type II keratins are clustered in a region of chromosome 12q13
Other Designations
keratin 1B
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Interactome
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Publication Reference
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Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
J Woodcock-Mitchell, R Eichner, W G Nelson, T T Sun.
The Journal of Cell Biology 1982 Nov; 95(2 Pt 1):580.
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Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies.
S C Tseng, M J Jarvinen, W G Nelson, J W Huang, J Woodcock-Mitchell, T T Sun.
Cell 1982 Sep; 30(2):361.
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Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
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