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Actin (muscle specific) monoclonal antibody, clone MSA/953

  • Catalog # : MAB14633
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of human muscle actin.
  • Immunogen:
  • A synthetic peptide corresponding to human muscle actins.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Rat
  • Specificity:
  • This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium.
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
    Immunofluorescence (0.5-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS (0.05% BSA and 0.05% azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of hSKM cell lysate with Actin (muscle specific) monoclonal antibody, clone MSA/953 (Cat # MAB14633).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human rhabdomyosarcoma (B) human leiomyosarcoma (C) rat lung (D) rat heart (E) rat stomach and (F) rat skeletal muscle with Actin (muscle specific) monoclonal antibody, clone MSA/953 (Cat # MAB14633).
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 58
  • Protein Accession#:
  • P62736; P68032; P68133
  • Gene Name:
  • ACTA1
  • Gene Alias:
  • ACTA,ASMA,CFTD,CFTD1,CFTDM,MPFD,NEM1,NEM2,NEM3
  • Gene Description:
  • actin, alpha 1, skeletal muscle
  • Gene Summary:
  • The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000036123,alpha skeletal muscle actin
  • Gene Information
  • Entrez GeneID:
  • 59
  • Protein Accession#:
  • P62736; P68032; P68133
  • Gene Name:
  • ACTA2
  • Gene Alias:
  • AAT6,ACTSA
  • Gene Description:
  • actin, alpha 2, smooth muscle, aorta
  • Gene Summary:
  • The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020042,alpha 2 actin,alpha-cardiac actin,growth-inhibiting gene 46
  • Gene Information
  • Entrez GeneID:
  • 70
  • Protein Accession#:
  • P62736; P68032; P68133
  • Gene Name:
  • ACTC1
  • Gene Alias:
  • ACTC,CMD1R,CMH11
  • Gene Description:
  • actin, alpha, cardiac muscle 1
  • Gene Summary:
  • Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq
  • Other Designations:
  • cardiac muscle alpha actin 1
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