Mouse monoclonal antibody raised against partial recombinant human TNF.
Immunogen:
Recombinant protein corresponding to N-terminus of human TNF.
Host:
Mouse
Theoretical MW (kDa):
17
Reactivity:
Human, Rat
Form:
Liquid
Purification:
Protein A/G purification
Isotype:
IgM, kappa
Recommend Usage:
Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 min a 95°C followed by cooling at RT for 20 minutes) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 10 mM PBS (0.05% sodium azide).
Storage Instruction:
Store at 4°C.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human Erdheim Chester disease (also known as polyostotic lerosing histiocytosis) and (B) rat pancreas with TNF monoclonal antibody, clone TNF706 (Cat # MAB14620).
Immunofluorescence
Immunofluorescence staining of HepG2 cells using this antibody followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with Reddot.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations:
APC1 protein,OTTHUMP00000029281,OTTHUMP00000037669,TNF superfamily, member 2,TNF, macrophage-derived,TNF, monocyte-derived,cachectin,tumor necrosis factor alpha