KRT7 monoclonal antibody, clone LDS-68
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Specification
Product Description
Mouse monoclonal antibody raised against native KRT7.
Immunogen
Native purified human KRT7.
Host
Mouse
Reactivity
Human
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
Immunohistochemistry (Frozen sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 1.2% sodium acetate (2 mg BSA, 0.01 mg sodium azide)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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Gene Info — KRT7
Entrez GeneID
3855Gene Name
KRT7
Gene Alias
CK7, K2C7, K7, MGC129731, MGC3625, SCL
Gene Description
keratin 7
Omim ID
148059Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq
Other Designations
cytokeratin 7|keratin, 55K type II cytoskeletal|keratin, simple epithelial type I, K7|keratin, type II cytoskeletal 7|sarcolectin|type II mesothelial keratin K7
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Interactome
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Publication Reference
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Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse.
Smith FJ, Porter RM, Corden LD, Lunny DP, Lane EB, McLean WH.
Biochemical and Biophysical Research Communications 2002 Oct; 297(4):818.
Application:WB-Ti, Human, Mouse, Bladder, Skin.
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Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12.
Rosenberg M, Fuchs E, Le Beau MM, Eddy RL, Shows TB.
Cytogenetics and Cell Genetics 1991 Jan; 57(1):33.
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Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse.
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