Mouse monoclonal antibody raised against native pig NEFH.
Crude neurofilament preparation from porcine spinal cord.
Theoretical MW (kDa):
Human, Pig, Rat
This monoclonal antibody identified as heavy subunit of neurofilaments (NEFH). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this antibody no longer binds.
Protein A/G purification
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL) The optimal working dilution should be determined by the end user.
In 10 mM PBS.
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.