NEFH (phospho KSP/KEP) monoclonal antibody, clone NE14
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum (A) and rat cerebellum (B) with NEFH (phospho KSP/KEP) monoclonal antibody, clone NE14 (Cat # MAB14382).
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Specification
Product Description
Mouse monoclonal antibody raised against native pig NEFH.
Immunogen
Crude neurofilament preparation from porcine spinal cord.
Host
Mouse
Theoretical MW (kDa)
200
Reactivity
Human, Pig, Rat
Specificity
This monoclonal antibody identified as heavy subunit of neurofilaments (NEFH). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this antibody no longer binds.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum (A) and rat cerebellum (B) with NEFH (phospho KSP/KEP) monoclonal antibody, clone NE14 (Cat # MAB14382).Flow Cytometry
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