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NEFH (phospho KSP/KEP) monoclonal antibody, clone NE14

  • Catalog # : MAB14381
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native pig NEFH.
  • Immunogen:
  • Crude neurofilament preparation from porcine spinal cord.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 200
  • Reactivity:
  • Human, Pig, Rat
  • Specificity:
  • This monoclonal antibody identified as heavy subunit of neurofilaments (NEFH). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this antibody no longer binds.
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum (A) and rat cerebellum (B) with NEFH (phospho KSP/KEP) monoclonal antibody, clone NE14 (Cat # MAB14381).
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Flow Cytometry
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