NCL monoclonal antibody, clone NCL/902
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human NCL.
Immunogen
Recombinant protein corresponding to full length human NCL.
Host
Mouse
Theoretical MW (kDa)
76
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western Blot analysis of A431 cell lysate with NCL monoclonal antibody, clone NCL/902 (Cat # MAB14380).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil (A), human skin (B), human uterus (C), human bladder carcinoma (D) and human ovarian carcinoma (E) with NCL monoclonal antibody, clone NCL/902 (Cat # MAB14380).Immunofluorescence
Immunofluorescent staining of HeLa cells with NCL monoclonal antibody, clone NCL/902 (Cat # MAB14380) (Green). Blue: nuclei.Flow Cytometry
Flow cytometric analysis of 293T cells (A) and Jurkat cells (B) with NCL monoclonal antibody, clone NCL/902 (Cat # MAB14380). A: Cells alone was black; isotype control was grey and AF488 conjugated format of NCL monoclonal antibody, clone NCL/902 was green. B: isotype control was grey; FITC conjugated format was green; APC conjugated format was purple and AF700 conjugated format of NCL monoclonal antibody, clone NCL/902 was turquoise. -
Gene Info — NCL
Entrez GeneID
4691Protein Accession#
P19338Gene Name
NCL
Gene Alias
C23, FLJ45706
Gene Description
nucleolin
Omim ID
164035Gene Ontology
HyperlinkGene Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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