Mouse monoclonal antibody raised against full length recombinant human NCL.
Recombinant protein corresponding to full length human NCL.
Theoretical MW (kDa):
Protein A/G purification
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In 10 mM PBS.
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil (A), human skin (B), human uterus (C), human bladder carcinoma (D) and human ovarian carcinoma (E) with NCL monoclonal antibody, clone NCL/902 (Cat # MAB14380).
Immunofluorescent staining of HeLa cells with NCL monoclonal antibody, clone NCL/902 (Cat # MAB14380) (Green). Blue: nuclei.
Flow cytometric analysis of 293T cells (A) and Jurkat cells (B) with NCL monoclonal antibody, clone NCL/902 (Cat # MAB14380). A: Cells alone was black; isotype control was grey and AF488 conjugated format of NCL monoclonal antibody, clone NCL/902 was green. B: isotype control was grey; FITC conjugated format was green; APC conjugated format was purple and AF700 conjugated format of NCL monoclonal antibody, clone NCL/902 was turquoise.
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq