ECM1 monoclonal antibody, clone ECM1/792
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma with ECM1 monoclonal antibody, clone ECM1/792 (Cat # MAB14251).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human ECM1.
Immunogen
Recombinant protein corresponding to full length human ECM1.
Host
Mouse
Theoretical MW (kDa)
~80
Reactivity
Human
Specificity
This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA, 0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma with ECM1 monoclonal antibody, clone ECM1/792 (Cat # MAB14251).Immunofluorescence
Flow Cytometry
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Gene Info — ECM1
Entrez GeneID
1893Protein Accession#
Q16610Gene Name
ECM1
Gene Alias
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Gene Description
extracellular matrix protein 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an extracellular protein containing motifs with a cysteine pattern characteristic of the cysteine pattern of the ligand-binding "double-loop" domains of the albumin protein family. This gene maps outside of the epidermal differentiation complex (EDC), a cluster of three gene families involved in epidermal differentiation. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000014932|OTTHUMP00000014934|secretory component p85
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Interactome
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Disease
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