Phosphotyrosine monoclonal antibody, clone E10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of tyrosine phosphorylation status in different cells with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
Lane 1: EGF-stimulated A431 cell lysates (50 ug)
Lane 2: Untreated A431 cell lysates (50 ug)
Lane 3: Insulin-stimulated HEK293 cell lysates (50 ug)
Lane 4: Untreated HEK293 cell lysates (50 ug)
Western Blot (Cell lysate)
Western blot analysis of a panel of phosphotyrosine antibodies with EGF-stimulated A431 cell lysates.
Lane 1: Phosphotyrosine monoclonal antibody, clone 5E10E1 (Cat # MAB14227)
Lane 2: Phosphotyrosine monoclonal antibody, clone 18E10D2 (Cat # MAB14228)
Lane 3: Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229)
Immunofluorescence
Immunofluorescent staining of EGF-stimulated A431 cells and untreated A431 cells with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of various phosphotyrosine peptides and corresponding non-phosphorylated peptides with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
Flow Cytometry
Flow cytometric analysis of EGF-stimulated A431 cells (red) and untreated A431 cells (black) with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
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Specification
Product Description
Mouse monoclonal antibody raised against Phosphotyrosine.
Immunogen
Phosphotyrosine containing carrier protein.
Host
Mouse
Specificity
The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.
Form
Lyophilized
Purification
Protein A purification
Isotype
IgG1, kappa; IgG2a, kappa
Recommend Usage
ELISA (0.1 ug/mL)
Flow Cytometry (4 ug/mL)
Immunocytochemistry (4 ug/mL)
Immunofluorescence (4 ug/mL)
Immunoprecipitation (1-4 ug/mL)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C or lower.
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of tyrosine phosphorylation status in different cells with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
Lane 1: EGF-stimulated A431 cell lysates (50 ug)
Lane 2: Untreated A431 cell lysates (50 ug)
Lane 3: Insulin-stimulated HEK293 cell lysates (50 ug)
Lane 4: Untreated HEK293 cell lysates (50 ug)Western Blot (Cell lysate)
Western blot analysis of a panel of phosphotyrosine antibodies with EGF-stimulated A431 cell lysates.
Lane 1: Phosphotyrosine monoclonal antibody, clone 5E10E1 (Cat # MAB14227)
Lane 2: Phosphotyrosine monoclonal antibody, clone 18E10D2 (Cat # MAB14228)
Lane 3: Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229)Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of EGF-stimulated A431 cells and untreated A431 cells with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of various phosphotyrosine peptides and corresponding non-phosphorylated peptides with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).Flow Cytometry
Flow cytometric analysis of EGF-stimulated A431 cells (red) and untreated A431 cells (black) with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
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