Mouse monoclonal antibody raised against Phosphotyrosine.
Phosphotyrosine containing carrier protein.
The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.
Protein A purification
IgG1, kappa; IgG2a, kappa
ELISA (0.1 ug/mL) Flow Cytometry (4 ug/mL) Immunocytochemistry (4 ug/mL) Immunofluorescence (4 ug/mL) Immunoprecipitation (1-4 ug/mL) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
Store at -20°C or lower. Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL. The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of a panel of phosphotyrosine antibodies with EGF-stimulated A431 cell lysates. Lane 1: Phosphotyrosine monoclonal antibody, clone 5E10E1 (Cat # MAB14227) Lane 2: Phosphotyrosine monoclonal antibody, clone 18E10D2 (Cat # MAB14228) Lane 3: Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229)
Western Blot (Cell lysate)
Western blot analysis of tyrosine phosphorylation status in different cells with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229). Lane 1: EGF-stimulated A431 cell lysates (50 ug) Lane 2: Untreated A431 cell lysates (50 ug) Lane 3: Insulin-stimulated HEK293 cell lysates (50 ug) Lane 4: Untreated HEK293 cell lysates (50 ug)
Immunofluorescent staining of EGF-stimulated A431 cells and untreated A431 cells with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
ELISA analysis of various phosphotyrosine peptides and corresponding non-phosphorylated peptides with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
Flow cytometric analysis of EGF-stimulated A431 cells (red) and untreated A431 cells (black) with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).