Product Browser

Last updated: 2017/2/19

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

Phosphotyrosine monoclonal antibody, clone E10

  • Catalog # : MAB14229
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Phosphotyrosine.
  • Immunogen:
  • Phosphotyrosine containing carrier protein.
  • Host:
  • Mouse
  • Specificity:
  • The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.
  • Form:
  • Lyophilized
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa; IgG2a, kappa
  • Recommend Usage:
  • ELISA (0.1 ug/mL)
    Flow Cytometry (4 ug/mL)
    Immunocytochemistry (4 ug/mL)
    Immunofluorescence (4 ug/mL)
    Immunoprecipitation (1-4 ug/mL)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C or lower.
    Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
    The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of a panel of phosphotyrosine antibodies with EGF-stimulated A431 cell lysates.
    Lane 1: Phosphotyrosine monoclonal antibody, clone 5E10E1 (Cat # MAB14227)
    Lane 2: Phosphotyrosine monoclonal antibody, clone 18E10D2 (Cat # MAB14228)
    Lane 3: Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of tyrosine phosphorylation status in different cells with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
    Lane 1: EGF-stimulated A431 cell lysates (50 ug)
    Lane 2: Untreated A431 cell lysates (50 ug)
    Lane 3: Insulin-stimulated HEK293 cell lysates (50 ug)
    Lane 4: Untreated HEK293 cell lysates (50 ug)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of EGF-stimulated A431 cells and untreated A431 cells with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of various phosphotyrosine peptides and corresponding non-phosphorylated peptides with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of EGF-stimulated A431 cells (red) and untreated A431 cells (black) with Phosphotyrosine monoclonal antibody, clone E10 (Cat # MAB14229).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Immunoprecipitation
  • RSS
  • YouTube
  • Linkedin
  • Facebook