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Last updated: 2017/4/23

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Phosphotyrosine monoclonal antibody, clone 5E10E1

  • Catalog # : MAB14227
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Phosphotyrosine.
  • Immunogen:
  • Phosphotyrosine containing carrier protein.
  • Host:
  • Mouse
  • Specificity:
  • The antibody is specific for proteins containing phosphotyrosine residues. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.
  • Form:
  • Lyophilized
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.5 mg/mL PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C or lower.
    Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
    The reconstituted antibody can be stored for 2-3 weeks at 4°C. For long term storage, aliquot and store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: EGF-stimulated A431, Lane 2: untreated A431 cell lysates with Phosphotyrosine monoclonal antibody, clone 5E10E1 (Cat # MAB14227).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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