Western Blot analysis of Lane 1: VEGFA transfected 293T cell culture supernatant and Lane 2: recombinant human VEGFA with VEGFA monoclonal antibody, clone 16F1 (Cat # MAB14190).
Immunofluorescent staining of HeLa cells (A) with VEGFA monoclonal antibody, clone 16F1 (Cat # MAB14190) (Green) compared to a negative control without primary antibody (B). Blue: nuclei. Red: F-actin.
Sandwich ELISA analysis of human VEGFA recombinant protein with VEGFA monoclonal antibody, clone 16F1 (Cat # MAB14190).
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq