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VAMP1 monoclonal antibody, clone SP10

  • Catalog # : MAB14169
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human VAMP1.
  • Immunogen:
  • Synaptic immunoprecipitate (crude) from human brain.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Purified
  • Isotype:
  • IgM
  • Recommend Usage:
  • Flow Cytometry (3-5 ug x 106 cells)
    Immunocytochemistry (2 ug/mL)
    Immunofluorescence (2 ug/mL)
    Immunohistochemistry
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.01 M HEPES, 0.15 M NaCl, pH 7.5 (50% glycerol, 100 ug/mL BSA).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of Lane 1: mouse cerebellum, Lane 2: NTERA-2, Lane 3: mouse brain, Lane 4: rat brain, Lane 5: SH-SY5Y and Lane 6: HeLa lysates with VAMP1 monoclonal antibody, clone SP10 (Cat # MAB14169).
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of SH-SY5Y cells with VAMP1 monoclonal antibody, clone SP10 (Cat # MAB14169). Panel a: VAMP1 (Green). Panel b: nuclei (Blue). Panel c: F-actin (Red). Panel d: merge. Panel e: no primary antibody control.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of SH-SY5Y cells with VAMP1 monoclonal antibody, clone SP10 (Cat # MAB14169) (Red histogram). Pink histogram: mouse isotype control. Purple histogram: unstained control cells. Green histogram: no primary antibody control.
  • Application Image
  • Immunohistochemistry
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 6843
  • Gene Name:
  • VAMP1
  • Gene Alias:
  • DKFZp686H12131,SYB1,VAMP-1
  • Gene Description:
  • vesicle-associated membrane protein 1 (synaptobrevin 1)
  • Gene Summary:
  • Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq
  • Other Designations:
  • synaptobrevin 1,vesicle-associated membrane protein 1
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