Mouse monoclonal antibody raised against human VAMP1.
Synaptic immunoprecipitate (crude) from human brain.
Human, Mouse, Rat
Flow Cytometry (3-5 ug x 106 cells) Immunocytochemistry (2 ug/mL) Immunofluorescence (2 ug/mL) Immunohistochemistry Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In 0.01 M HEPES, 0.15 M NaCl, pH 7.5 (50% glycerol, 100 ug/mL BSA).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Western Blot analysis of Lane 1: mouse cerebellum, Lane 2: NTERA-2, Lane 3: mouse brain, Lane 4: rat brain, Lane 5: SH-SY5Y and Lane 6: HeLa lysates with VAMP1 monoclonal antibody, clone SP10 (Cat # MAB14169).
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq
synaptobrevin 1,vesicle-associated membrane protein 1