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STAT1 monoclonal antibody, clone 15H3

  • Catalog # : MAB14168
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human STAT1.
  • Immunogen:
  • Protein expressed in 293T cell transfected with human STAT1 expression vector.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Monkey
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry (1:20)
    Immunofluorescence (1:20)
    Immunoprecipitation (2 ug)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: A549, Lane 2: 293T, Lane 3: Jurkat, Lane 4: A431, Lane 5: U2OS, Lane 6: COS7 and Lane 7: 3T3 L1 cell lysates with STAT1 monoclonal antibody, clone 15H3 (Cat # MAB14168).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa (A), MCF-7 (B) and A2058 (C) cells (left) with STAT1 monoclonal antibody, clone 15H3 (Cat # MAB14168) (Green) compared to a negative control without primary antibody respectively (right). Blue: nuclei. Red: F-actin.
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation analysis of U2OS cells with STAT1 monoclonal antibody, clone 15H3 (Cat # MAB14168). Lane 1: lysate. Lane 2: IP.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 6772
  • Gene Name:
  • STAT1
  • Gene Alias:
  • DKFZp686B04100,ISGF-3,STAT91
  • Gene Description:
  • signal transducer and activator of transcription 1, 91kDa
  • Gene Summary:
  • The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000165047,signal transducer and activator of transcription 1,signal transducer and activator of transcription-1,transcription factor ISGF-3 components p91/p84
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