Mouse monoclonal antibody raised against full length recombinant human SOD2.
Recombinant protein corresponding to full length human SOD2.
Protein A affinity purification
Flow Cytometry (3-5 ug x 106 cells) Immunocytochemistry (2 ug/mL) Immunofluorescence (2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:200) Immunoprecipitation (2 ug) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: 293T, Lane 2: HeLa, Lane 3: HepG2, Lane 4: MDCK, Lane 5: 3T3, Lane 6: NRK, Lane 7: A431, Lane 8: U2OS, Lane 9: K562 and Lane 10: MEF cell lysates with SOD2 monoclonal antibody, clone 1H6 (Cat # MAB14166).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the mitochondrion matrix of human liver (A) and human cervical carcinoma (B) tissues (right) with SOD2 monoclonal antibody, clone 1H6 (Cat # MAB14166) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining in the mitochondrion matrix of HeLa (A) and HUVEC (B) cells (right) with SOD2 monoclonal antibody, clone 1H6 (Cat # MAB14166) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.
Immunoprecipitation analysis of HepG2 cells with SOD2 monoclonal antibody, clone 1H6 (Cat # MAB14166). Lane 1: lysate. Lane 2: IP.
Flow cytometric analysis of HeLa cells with SOD2 monoclonal antibody, clone 1H6 (Cat # MAB14166) (Red histogram). Pink histogram: mouse isotype control. Purple histogram: unstained control cells. Green histogram: no primary antibody control.
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq