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SOD1 monoclonal antibody, clone 8B10

  • Catalog # : MAB14165
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human SOD1.
  • Immunogen:
  • Recombinant protein corresponding to full length human SOD1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (2 ug/mL)
    Immunofluorescence (2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:200)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HepG2, Lane 2: MDCK, Lane 3: 3T3, Lane 4: NRK, Lane 5: K562 and Lane 6: MEF cell lysates with SOD1 monoclonal antibody, clone 8B10 (Cat # MAB14165).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm of human placenta (A) and in the nucleus and cytoplasm of human breast carcinoma (B) tissues (right) with SOD1 monoclonal antibody, clone 8B10 (Cat # MAB14165) compared to a negative control without primary antibody respectively (left).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining in the cytoplasm and nucleus of HeLa (A), HepG2 (B) and 3T3-L1 (C) cells (right) with SOD1 monoclonal antibody, clone 8B10 (Cat # MAB14165) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 6647
  • Gene Name:
  • SOD1
  • Gene Alias:
  • ALS,ALS1,IPOA,SOD,homodimer
  • Gene Description:
  • superoxide dismutase 1, soluble
  • Gene Summary:
  • The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq
  • Other Designations:
  • Cu /Zn superoxide dismutase,Cu/Zn superoxide dismutase,SOD, soluble,indophenoloxidase A,superoxide dismutase, cystolic
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