Mouse monoclonal antibody raised against full length recombinant human RHOA/RHOC.
Recombinant protein corresponding to full length human RHOA.
Protein A affinity purification
Immunocytochemistry (1:20-1:200) Immunofluorescence (1:20-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:500) Immunoprecipitation (2 ug) Western Blot (1:500-1:1500) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: HeLa, Lane 2: A431, Lane 3: HEK293T, Lane 4: U2-OS, Lane 5: NIH/3T3 and Lane 6: K562 cell lysates, Lane 7: RHOA, Lane 8: RHOB and Lane 9: GST-RHOC purified proteins with RHOA/RHOC monoclonal antibody, clone 1B3-4A10 (Cat # MAB14156).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm of human hepatocarcinoma (A), in the cytoplasm and membrane of mouse liver (B) and human prostate carcinoma (C) tissues (right) with RHOA/RHOC monoclonal antibody, clone 1B3-4A10 (Cat # MAB14156) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining in the cytoplasm of HeLa (A) and A431 (B) cells (right) with RHOA/RHOC monoclonal antibody, clone 1B3-4A10 (Cat # MAB14156) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.
Immunoprecipitation analysis of HeLa cells with RHOA/RHOC monoclonal antibody, clone 1B3-4A10 (Cat # MAB14156). Lane 1: lysate. Lane 2: IP.
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
OTTHUMP00000013675,OTTHUMP00000013676,OTTHUMP00000013802,OTTHUMP00000013805,OTTHUMP00000013807,OTTHUMP00000013809,RAS-related homolog 9,Rho-related GTP-binding protein RhoC,oncogene RHO H9,rhoC GTPase,small GTP binding protein RhoC