Mouse monoclonal antibody raised against full length recombinant human RHOA/RHOB/RHOC.
Recombinant protein corresponding to full length human RHOA.
Protein A affinity purification
Immunocytochemistry (1:10-1:200) Immunofluorescence (1:10-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:200) Immunoprecipitation (2 ug) Western Blot (1:500-1:1500) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: HeLa, Lane 2: A431, Lane 3: HEK293T, Lane 4: U2-OS and Lane 5: NIH3T3 cell lysates, Lane 6: full-length His-RHOA, Lane 7: truncated GST-RHOB and Lane 8: full-length GST-RHOC purified proteins with RHOA/RHOB/RHOC monoclonal antibody, clone 1A11-4G10 (Cat # MAB14155).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm of mouse breast (A), human hepatocarcinoma (B) and human prostate carcinoma (C) tissues (right) compared to a negative control without primary antibody respectively (left) with RHOA/RHOB/RHOC monoclonal antibody, clone 1A11-4G10 (Cat # MAB14155).
Immunofluorescent staining in the cytoplasm of NIH-3T3 (A) and A431 (B) cells (right) compared to a negative control without primary antibody respectively (left) with RHOA/RHOB/RHOC monoclonal antibody, clone 1A11-4G10 (Cat # MAB14155) (Green). Blue: nuclei.
Immunoprecipitation analysis of HeLa cells with RHOA/RHOB/RHOC monoclonal antibody, clone 1A11-4G10 (Cat # MAB14155). Lane 1: lysate. Lane 2: IP.
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
OTTHUMP00000013675,OTTHUMP00000013676,OTTHUMP00000013802,OTTHUMP00000013805,OTTHUMP00000013807,OTTHUMP00000013809,RAS-related homolog 9,Rho-related GTP-binding protein RhoC,oncogene RHO H9,rhoC GTPase,small GTP binding protein RhoC