Mouse monoclonal antibody raised against full length recombinant human RAP1A.
Recombinant protein corresponding to full length human RAP1A.
Protein A affinity purification
Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:500) Immunoprecipitation (2 ug) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm and membrane of human kidney (A) and human colon carcinoma (B) tissues (right) with RAP1A monoclonal antibody, clone 1D4-1A3 (Cat # MAB14149) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining of HeLa cells with RAP1A monoclonal antibody, clone 1D4-1A3 (Cat # MAB14149) (Green). Blue: nuclei.
Immunoprecipitation analysis of NIH/3T3 cells with RAP1A monoclonal antibody, clone 1D4-1A3 (Cat # MAB14149). Lane 1: lysate. Lane 2: IP.
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
OTTHUMP00000013740,OTTHUMP00000013741,OTTHUMP00000059089,RAS-related protein RAP1A,Ras-related protein Krev-1,Ras-related protein Rap-1A