Product Browser

Last updated: 2017/5/21

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

RAP1A monoclonal antibody, clone 1D4-1A3

  • Catalog # : MAB14149
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human RAP1A.
  • Immunogen:
  • Recombinant protein corresponding to full length human RAP1A.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:500)
    Immunoprecipitation (2 ug)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HeLa, Lane 2: HEK293T, Lane 3: U2OS and Lane 4: NIH3T3 cell lysates with RAP1A monoclonal antibody, clone 1D4-1A3 (Cat # MAB14149).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm and membrane of human kidney (A) and human colon carcinoma (B) tissues (right) with RAP1A monoclonal antibody, clone 1D4-1A3 (Cat # MAB14149) compared to a negative control without primary antibody respectively (left).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cells with RAP1A monoclonal antibody, clone 1D4-1A3 (Cat # MAB14149) (Green). Blue: nuclei.
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation analysis of NIH/3T3 cells with RAP1A monoclonal antibody, clone 1D4-1A3 (Cat # MAB14149). Lane 1: lysate. Lane 2: IP.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 5906
  • Gene Name:
  • RAP1A
  • Gene Alias:
  • KREV-1,KREV1,RAP1,SMGP21
  • Gene Description:
  • RAP1A, member of RAS oncogene family
  • Gene Summary:
  • The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000013740,OTTHUMP00000013741,OTTHUMP00000059089,RAS-related protein RAP1A,Ras-related protein Krev-1,Ras-related protein Rap-1A
  • RSS
  • YouTube
  • Linkedin
  • Facebook