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PLK1 monoclonal antibody, clone PL6/PL2

  • Catalog # : MAB14141
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human PLK1.
  • Immunogen:
  • Recombinant His fusion protein corresponding to amino acids 402-603 at C-terminus of human PLK1.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG1, kappa; IgG2b
  • Recommend Usage:
  • ELISA (0.1-1 ug/mL)
    Flow Cytometry (3-5 ug x 106 cells)
    Immunocytochemistry (1-5 ug/mL)
    Immunofluorescence (1-5 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-1:100)
    Immunoprecipitation (5 ug)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of A431 cells with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141).
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HEK-293, Lane 2: K562, Lane 3: A549, Lane 4: HT-29, Lane 5: COLO 205, Lane 6: PC-12, Lane 7: Cos-7, Lane 8: NIH3T3, Lane 9: A-431 and Lane 10: HeLa cell lysates with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the nucleus and cytoplasm of human colon (A) and mouse colon (B) tissues (right) with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141) compared to a negative control without primary antibody respectively (left).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cells with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141). Panel a: PLK1 (Green). Panel b: nuclei (Blue). Panel c: F-actin (Red). Panel d: merge. Panel e: no primary antibody control.
  • Immunoprecipitation
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K562 cells with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141) (Red histogram). Pink histogram: mouse isotype control. Purple histogram: unstained control cells. Green histogram: no primary antibody control.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5347
  • Gene Name:
  • PLK1
  • Gene Alias:
  • PLK,STPK13
  • Gene Description:
  • polo-like kinase 1 (Drosophila)
  • Other Designations:
  • cell cycle regulated protein kinase,polo (Drosophia)-like kinase,polo like kinase,polo-like kinase
  • Interactome
  • Interactome
  • Gene Pathway
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