Mouse monoclonal antibody raised against partial recombinant human PLK1.
Recombinant His fusion protein corresponding to amino acids 402-603 at C-terminus of human PLK1.
Protein A affinity purification
IgG1, kappa; IgG2b
ELISA (0.1-1 ug/mL) Flow Cytometry (3-5 ug x 106 cells) Immunocytochemistry (1-5 ug/mL) Immunofluorescence (1-5 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-1:100) Immunoprecipitation (5 ug) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of A431 cells with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141).
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HEK-293, Lane 2: K562, Lane 3: A549, Lane 4: HT-29, Lane 5: COLO 205, Lane 6: PC-12, Lane 7: Cos-7, Lane 8: NIH3T3, Lane 9: A-431 and Lane 10: HeLa cell lysates with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the nucleus and cytoplasm of human colon (A) and mouse colon (B) tissues (right) with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining of HeLa cells with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141). Panel a: PLK1 (Green). Panel b: nuclei (Blue). Panel c: F-actin (Red). Panel d: merge. Panel e: no primary antibody control.
Flow cytometric analysis of K562 cells with PLK1 monoclonal antibody, clone PL6/PL2 (Cat # MAB14141) (Red histogram). Pink histogram: mouse isotype control. Purple histogram: unstained control cells. Green histogram: no primary antibody control.