Mouse monoclonal antibody raised against partial recombinant human PAX8.
Recombinant protein corresponding to internal region of human PAX8.
Protein A affinity purification
Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:200) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the nucleus of mouse kidney (A), human kidney (B) and human thyroid (C) tissues (right) with PAX8 monoclonal antibody, clone 1F8-3A8 (Cat # MAB14135) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining shows specific expression in COS7 cells (A, shown in green) but not in negative control 293T cells (B) with PAX8 monoclonal antibody, clone 1F8-3A8 (Cat # MAB14135). Blue: nuclei. Red: F-actin.
This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq