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PAX8 monoclonal antibody, clone 1F8-3A8

  • Catalog # : MAB14135
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human PAX8.
  • Immunogen:
  • Recombinant protein corresponding to internal region of human PAX8.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:200)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: empty vector control and Lane 2: HEK293T cell lysate overexpressing PAX8 with PAX8 monoclonal antibody, clone 1F8-3A8 (Cat # MAB14135).
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: SKOV-3, Lane 2: COS7, and Lane 3: HeLa (negative control) cell lysates with PAX8 monoclonal antibody, clone 1F8-3A8 (Cat # MAB14135).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the nucleus of mouse kidney (A), human kidney (B) and human thyroid (C) tissues (right) with PAX8 monoclonal antibody, clone 1F8-3A8 (Cat # MAB14135) compared to a negative control without primary antibody respectively (left).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining shows specific expression in COS7 cells (A, shown in green) but not in negative control 293T cells (B) with PAX8 monoclonal antibody, clone 1F8-3A8 (Cat # MAB14135). Blue: nuclei. Red: F-actin.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 7849
  • Gene Name:
  • PAX8
  • Gene Alias:
  • -
  • Gene Description:
  • paired box 8
  • Gene Summary:
  • This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000158659,OTTHUMP00000158660,paired domain gene 8
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