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MAPK3 monoclonal antibody, clone 12D11

  • Catalog # : MAB14131
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human MAPK3.
  • Immunogen:
  • Recombinant protein corresponding to full length human MAPK3.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Monkey, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ChIP (1-3 uL)
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • Chromatin Immunoprecipitation (ChIP) analysis of HCT116 cells treated with serum using MAPK3 monoclonal antibody, clone 12D11 (Cat # MAB14131). A schematic representation of the EGR-1 locus is shown above the data where boxes represent exons (black boxes is translated regions, white boxes is untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by EGR-1 primers are represented by black bars.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: 293T, Lane 2: U2-OS, Lane 3: K562, Lane 4: A549, Lane 5: HeLa, Lane 6: Jurkat, Lane 7: MCF7, Lane 8: HepG2, Lane 9: MEF, Lane 10: NIH3T3, Lane 11: C2C12 and Lane 12: COS7 cell lysates with MAPK3 monoclonal antibody, clone 12D11 (Cat # MAB14131).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with MAPK3 monoclonal antibody, clone 12D11 (Cat # MAB14131).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cells with MAPK3 monoclonal antibody, clone 12D11 (Cat # MAB14131) (Green). Blue: nuclei.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 5595
  • Gene Name:
  • MAPK3
  • Gene Alias:
  • ERK1,HS44KDAP,HUMKER1A,MGC20180,P44ERK1,P44MAPK,PRKM3
  • Gene Description:
  • mitogen-activated protein kinase 3
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174538,OTTHUMP00000174540,extracellular signal-regulated kinase 1,extracellular signal-related kinase 1
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