Mouse monoclonal antibody raised against human MAPK1.
Protein expressed in 293T transfected with human MAPK1 expression vector.
Protein A affinity purification
ChIP (1-3 uL) Flow Cytometry (1-10 ug/mL) Immunocytochemistry (1:200) Immunofluorescence (1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:500) Immunoprecipitation (4 ug/mL) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Chromatin Immunoprecipitation (ChIP) analysis of HCT116 cells treated with serum using MAPK1 monoclonal antibody, clone 6F8 (Cat # MAB14130). A schematic representation of the EGR-1 locus is shown above the data where boxes represent exons (black boxes is translated regions, white boxes is untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by EGR-1 primers are represented by black bars.
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: 293T, Lane 3: HepG2, Lane 4: A549, Lane 5: MCF7, Lane 6: NIH/3T3, Lane 7: NRK, Lane 8: COS7 and Lane 9: MDCK cell lysates with MAPK1 monoclonal antibody, clone 6F8 (Cat # MAB14130).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of mouse skeletal muscle (A), human skeletal muscle (B) and human breast carcinoma (C) tissues (right) with MAPK1 monoclonal antibody, clone 6F8 (Cat # MAB14130) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining of HeLa cells with MAPK1 monoclonal antibody, clone 6F8 (Cat # MAB14130) (Green). Blue: nuclei.
Flow cytometric analysis of HeLa cells with MAPK1 monoclonal antibody, clone 6F8 (Cat # MAB14130) (Blue histogram). Red histogram: mouse IgG2a isotype control.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq