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POU5F1 monoclonal antibody, clone 9B7

  • Catalog # : MAB14128
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human POU5F1.
  • Immunogen:
  • Recombinant protein corresponding to full length human POU5F1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (3-5 ug x 106 cells)
    Immunocytochemistry (1-2 ug/mL)
    Immunofluorescence (1-2 ug/mL)
    Western Blot (1:500-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: NCCIT, Lane 2: NTERA-2, Lane 3: F9 and Lane 4: HeLa cell lysates with POU5F1 monoclonal antibody, clone 9B7 (Cat # MAB14128).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of NTREA-2 cells with POU5F1 monoclonal antibody, clone 9B7 (Cat # MAB14128). Panel a: POU5F1 (Green). Panel b: nuclei (Blue). Panel c: F-actin (Red). Panel d: merge. Panel e: no primary antibody control.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of human NCCIT (A) and A375 (B) cells with POU5F1 monoclonal antibody, clone 9B7 (Cat # MAB14128) (Red histogram). Blue histogram: PBS alone (A). Yellow histogram: mouse isotype control, purple histogram: unstained control cells and green histogram: no primary antibody control (B).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 5460
  • Gene Name:
  • POU5F1
  • Gene Alias:
  • MGC22487,OCT3,OCT4,OTF3,OTF4
  • Gene Description:
  • POU class 5 homeobox 1
  • Other Designations:
  • OTTHUMP00000029292,POU domain, class 5, transcription factor 1,POU-type homeodomain-containing DNA-binding protein,octamer-binding transcription factor-3
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