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NANOG monoclonal antibody, clone 23D2-3C6

  • Catalog # : MAB14124
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant human NANOG.
  • Immunogen:
  • Recombinant protein corresponding to full length human NANOG.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry
    Immunocytochemistry (1:50-1:100)
    Immunofluorescence (1:50-1:100)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: NCCIT, Lane 2: NTERA-2 and Lane 3: HeLa cell lysates with NANOG monoclonal antibody, clone 23D2-3C6 (Cat # MAB14124).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining shows specific expression in NTERA-2 cells (A, shown in green) but not in negative control HeLa cells (B) with NANOG monoclonal antibody, clone 23D2-3C6 (Cat # MAB14124). Blue: nuclei. Red: F-actin.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of H9 embryonic stem cells (A) and HEL 11.4 induced IPS cells (B) with NANOG monoclonal antibody, clone 23D2-3C6 (Cat # MAB14124) (Blue histogram). Green histogram: mouse IgG.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Gene Name:
  • NANOG
  • Gene Alias:
  • -
  • Gene Description:
  • Nanog homeobox
  • Other Designations:
  • homeobox transcription factor Nanog,homeobox transcription factor Nanog-delta 48
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