Western Blot analysis of Lane 1: 293T, Lane 2: HeLa, Lane 3: NIH3T3, Lane 4: COS7, Lane 5: K562, Lane 6: U2OS, Lane 7: NRK and Lane 8: NB4 cell lysates with MAP2K4 monoclonal antibody, clone 2 E4 (Cat # MAB14121).
Immunofluorescent staining shows specific expression in HeLa cells (A, shown in green) but not in negative control NRK cells (B) with MAP2K4 monoclonal antibody, clone 2 E4 (Cat # MAB14121). Blue: nuclei. Red: F-actin.
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver. [provided by RefSeq