Mouse monoclonal antibody raised against human MAP2K1.
Protein expressed in 293T cells transfected with human MAP2K1 expression vector.
Protein A affinity purification
Flow Cytometry (5-20 ug/mL) Immunocytochemistry (1:200) Immunofluorescence (1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10000-1:100000) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the nucleus and cytoplasm of mouse bladder (A), human bladder carcinoma (B) and human cervical carcinoma (C) tissues (right) with MAP2K1 monoclonal antibody, clone 1C1 (Cat # MAB14120) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining in the cytoplasm of Hela (A), A431 (B) and NIH-3T3 (C) cells (right) with MAP2K1 monoclonal antibody, clone 1C1 (Cat # MAB14120) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.
Flow cytometric analysis of HeLa (A) and NIH-3T3 (B) cells with MAP2K1 monoclonal antibody, clone 1C1 (Cat # MAB14120) (Green and Orange histogram). Black histogram: mouse isotype control.
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)