Mouse monoclonal antibody raised against full length recombinant human LIN28.
Recombinant protein corresponding to full length human LIN28.
Human, Mouse, Rat
Protein A affinity purification
ChIP (1-3 uL) Flow Cytometry (1:100) Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:200) Immunoprecipitation (5 ug) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Chromatin Immunoprecipitation (ChIP) analysis of rat hepatoma cells treated with insulin using LIN28 monoclonal antibody, clone 14E6-4E6 (Cat # MAB14112). A schematic representation of the rat Egr1 locus is shown above the data where boxes represent exons (black boxes is translated regions, white boxes is untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr1 primers are represented by black bars.
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NCCIT, Lane 2: NTERA-2, Lane 3: F9 and Lane 4: HeLa cell lysates with LIN28 monoclonal antibody, clone 14E6-4E6 (Cat # MAB14112).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the nucleus and cytoplasm of mouse testis (A) and in the cytoplasm of human seminoma (B) tissues (right) with LIN28 monoclonal antibody, clone 14E6-4E6 (Cat # MAB14112) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining of HeLa (A) and NCCIT (B) cells with LIN28 monoclonal antibody, clone 14E6-4E6 (Cat # MAB14112) (Green). Blue: nuclei. Red: F-actin.
Immunoprecipitation analysis of NCCIT cell lysate with LIN28 monoclonal antibody, clone 14E6-4E6 (Cat # MAB14112). Lane 1: lysate. Lane 2: IP.
Flow cytometric analysis of H9 embryonic stem cells (A) and HEL 11.4 induced IPS cells (B) with LIN28 monoclonal antibody, clone 14E6-4E6 (Cat # MAB14112) (Blue histogram). Green histogram: mouse IgG.