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KRAS monoclonal antibody, clone 9.13

  • Catalog # : MAB14111
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of human KRAS.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human KRAS.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunofluorescence (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-1:100)
    Immunoprecipitation
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of Lane 1: HCT-116, Lane 2: K562, Lane 3: Jurkat, Lane 4: mouse colon and Lane 5: mouse stomach lysates with KRAS monoclonal antibody, clone 9.13 (Cat # MAB14111).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm of human colon carcinoma (A) and in the cytoplasm and membrane of human prostate carcinoma (B) tissues (right) with KRAS monoclonal antibody, clone 9.13 (Cat # MAB14111) compared to a negative control without primary antibody respectively (left).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of MCF-7 cells with KRAS monoclonal antibody, clone 9.13 (Cat # MAB14111). Panel a: KRAS (Green). Panel b: nuclei (Blue). Panel c: F-actin (Red). Panel d: merge. Panel e: no primary antibody control.
  • Immunoprecipitation
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 3845
  • Gene Name:
  • KRAS
  • Gene Alias:
  • C-K-RAS,K-RAS2A,K-RAS2B,K-RAS4A,K-RAS4B,KI-RAS,KRAS1,KRAS2,NS3,RASK2
  • Gene Description:
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
  • Gene Summary:
  • This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq
  • Other Designations:
  • K-ras p21 protein,Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog,PR310 c-K-ras oncogene,c-K-ras2 protein,c-Kirsten-ras protein,cellular c-Ki-ras2 proto-oncogene,oncogene KRAS2,transforming protein p21,v-Ki-ras2 Kirsten rat sarcoma 2 viral oncoge
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