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Last updated: 2016/9/25

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Mouse Anti-Human IgM secondary antibody

  • Catalog # : MAB14095
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human IgM.
  • Immunogen:
  • Purified Human Serum IgM.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1 ug/mL)
    Flow Cytometry (1 ug/mL)
    Immunoprecipitation
    Radioimmunoassay
    Western Blot (1:100-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.01 M potassium phosphate buffer, 2.5 mM KCl, 0.135 M NaCl (1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of Lane 1: purified human IgM, Lane 2: purified human IgA and Lane 3: purified human IgG with Mouse Anti-Human IgM secondary antibody (Cat # MAB14095).
  • Immunoprecipitation
  • ELISA
  • ELISA
  • ELISA analysis of human IgM, IgG, and IgA with Mouse Anti-Human IgM secondary antibody (Cat # MAB14095).
  • Radioimmunoassay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of surface IgM expression (A) and cytoplasmic IgM expression (B) on Ramos (surface and cytoplasmic IgG/lambda-expressing B cells), Raji (cytoplasmic IgG/kappa-expressing B cells), and Jurkat (T cells) cell lines with Mouse Anti-Human IgM secondary antibody (Cat # MAB14095) (Red histogram). Black histogram: mouse IgG1 isotype control.
  • Application Image
  • Immunoprecipitation
  • Radioimmunoassay
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