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Histone H3 monoclonal antibody, clone 865R2

  • Catalog # : MAB14088
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human Histone H3.
  • Immunogen:
  • Recombinant protein corresponding to human Histone H3.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Purified
  • Isotype:
  • IgM
  • Recommend Usage:
  • Flow Cytometry (3-5 ug x 106 cells)
    Immunocytochemistry (2 ug/mL)
    Immunofluorescence (2 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HT-29, Lane 2:A431 and Lane 3: COLO 205 cell lysates with Histone H3 monoclonal antibody, clone 865R2 (Cat # MAB14088).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cells with Histone H3 monoclonal antibody, clone 865R2 (Cat # MAB14088). Panel a: Histone H3 (Green). Panel b: nuclei (Blue). Panel c: F-actin (Red). Panel d: merge. Panel e: no primary antibody control.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells with Histone H3 monoclonal antibody, clone 865R2 (Cat # MAB14088) (Red histogram). Pink histogram: mouse isotype control. Purple histogram: unstained control cells. Green histogram: no primary antibody control.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
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