Mouse monoclonal antibody raised against recombinant human GATA3.
Recombinant protein corresponding to human GATA3.
Human, Mouse, Rat
Protein A affinity purification
Immunocytochemistry (1:50-1:100) Immunofluorescence (1:50-1:100) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:2000) Immunoprecipitation (5 ug) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the nucleus of rat brain (A), mouse brain (B) and human breast carcinoma (C) tissues (right) with GATA3 monoclonal antibody, clone 1A12-1D9 (Cat # MAB14065) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining of MCF7 (A) and SH-SY5Y (B) cells (right) with GATA3 monoclonal antibody, clone 1A12-1D9 (Cat # MAB14065) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.