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GATA3 monoclonal antibody, clone 1A12-1D9

  • Catalog # : MAB14065
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human GATA3.
  • Immunogen:
  • Recombinant protein corresponding to human GATA3.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry (1:50-1:100)
    Immunofluorescence (1:50-1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:2000)
    Immunoprecipitation (5 ug)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: empty vector control and Lane 2: HEK293T cell lysate overexpressing GATA3-DDK with GATA3 monoclonal antibody, clone 1A12-1D9 (Cat # MAB14065).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the nucleus of rat brain (A), mouse brain (B) and human breast carcinoma (C) tissues (right) with GATA3 monoclonal antibody, clone 1A12-1D9 (Cat # MAB14065) compared to a negative control without primary antibody respectively (left).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of MCF7 (A) and SH-SY5Y (B) cells (right) with GATA3 monoclonal antibody, clone 1A12-1D9 (Cat # MAB14065) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2625
  • Gene Name:
  • GATA3
  • Gene Alias:
  • HDR,MGC2346,MGC5199,MGC5445
  • Gene Description:
  • GATA binding protein 3
  • Other Designations:
  • GATA-binding protein 3,OTTHUMP00000019070,OTTHUMP00000019071
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