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Last updated: 2016/11/27

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FN1 monoclonal antibody, clone 3F12

  • Catalog # : MAB14053
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human FN1.
  • Immunogen:
  • Human plasma FN1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1 ug/mL)
    Immunocytochemistry (1:100-1:200)
    Immunofluorescence (1:100-1:200)
    Immunoprecipitation
    Radioimmunoassay
    Western Blot (1:100-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.01 M potassium phosphate buffer, 2.5 mM KCl, 0.135 M NaCl (1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western Blot analysis of human plasma with FN1 monoclonal antibody, clone 3F12 (Cat # MAB14053).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HepG2 cells (A) with FN1 monoclonal antibody, clone 3F12 (Cat # MAB14053) (Green) compared to a negative control without primary antibody (B). Blue: nuclei.
  • Immunoprecipitation
  • Sandwich ELISA
  • Sandwich ELISA
  • Sandwich ELISA analysis of human FN1 recombinant protein (A) and human serum or human plasma (B) with FN1 monoclonal antibody, clone 3F12 (Cat # MAB14053).
  • Radioimmunoassay
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunocytochemistry
  • Immunoprecipitation
  • Radioimmunoassay
  • Gene Information
  • Entrez GeneID:
  • 2335
  • Gene Name:
  • FN1
  • Gene Alias:
  • CIG,DKFZp686F10164,DKFZp686H0342,DKFZp686I1370,DKFZp686O13149,ED-B,FINC,FN,FNZ,GFND,GFND2,LETS,MSF
  • Gene Description:
  • fibronectin 1
  • Gene Summary:
  • This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq
  • Other Designations:
  • cold-insoluble globulin,migration-stimulating factor
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