Mouse monoclonal antibody raised against partial recombinant human MAPK6.
Recombinant protein corresponding to amino acids 345-721 of human MAPK6.
Protein A affinity purification
Immunocytochemistry (1:100) Immunofluorescence (1:100) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm of mouse skeletal muscle (A), human skeletal muscle (B) and in the cytoplasm and nucleus of human breast carcinoma (C) tissues (right) with MAPK6 monoclonal antibody, clone 5E1 (Cat # MAB14043) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining in the cytoplasm of HeLa (A), in the cytoplasm and nucleus of HT-29 (B) and NIH-3T3 (C) cells (right) with MAPK6 monoclonal antibody, clone 5E1 (Cat # MAB14043) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq