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MAPK6 monoclonal antibody, clone 5E1

  • Catalog # : MAB14043
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human MAPK6.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 345-721 of human MAPK6.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HeLa, Lane 2: 293T and Lane 3: 3T3 cell lysates with MAPK6 monoclonal antibody, clone 5E1 (Cat # MAB14043).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm of mouse skeletal muscle (A), human skeletal muscle (B) and in the cytoplasm and nucleus of human breast carcinoma (C) tissues (right) with MAPK6 monoclonal antibody, clone 5E1 (Cat # MAB14043) compared to a negative control without primary antibody respectively (left).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining in the cytoplasm of HeLa (A), in the cytoplasm and nucleus of HT-29 (B) and NIH-3T3 (C) cells (right) with MAPK6 monoclonal antibody, clone 5E1 (Cat # MAB14043) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 5597
  • Gene Name:
  • MAPK6
  • Gene Alias:
  • DKFZp686F03189,ERK3,HsT17250,PRKM6,p97MAPK
  • Gene Description:
  • mitogen-activated protein kinase 6
  • Gene Summary:
  • The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq
  • Other Designations:
  • MAP kinase isoform p97,extracellular signal-regulated kinase 3,extracellular signal-regulated kinase, p97,protein kinase, mitogen-activated 5,protein kinase, mitogen-activated 6
  • Gene Pathway
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