Mouse monoclonal antibody raised against human EIF2S1.
Full length human EIF2S1 produced in HEK293T cells.
Human, Monkey, Rat
Protein A affinity purification
Immunocytochemistry (1:20-1:200) Immunofluorescence (1:20-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:200) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In PBS (30% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: HeLa, Lane 2: K562, Lane 3: Jurkat, Lane 4: 293T, Lane 5: U2-OS, Lane 6: HepG2, Lane 7: COS7, Lane 8: NIH3T3 and Lane 9: NRK cell lysates with EIF2S1 monoclonal antibody, clone 5A5 (Cat # MAB14039).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm of human colon (A) and human breast carcinoma (B) tissues (right) with EIF2S1 monoclonal antibody, clone 5A5 (Cat # MAB14039) compared to a negative control without primary antibody respectively (left).
Immunofluorescent staining in the cytoplasm of HeLa (A), MCF-7 (B) and COS7 (C) cells (right) with EIF2S1 monoclonal antibody, clone 5A5 (Cat # MAB14039) (Green) compared to a negative control without primary antibody respectively (left). Blue: nuclei. Red: F-actin.
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM