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EGF monoclonal antibody, clone 3A8

  • Catalog # : MAB14035
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human EGF.
  • Immunogen:
  • Recombinant protein corresponding to human EGF.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This antibody has successfully been used as the detection antibody in a sandwich ELISA.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (0.125-0.25 ug/mL)
    Immunocytochemistry (1:50)
    Immunofluorescence (1:50)
    Western Blot (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS.
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of Lane 1: human EGF recombinant protein and Lane 2: supernatant from an EGF expression clone transfected in 293T cells with EGF monoclonal antibody, clone 3A8 (Cat # MAB14035).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of HeLa cells either untreated (A) or treated with EGF (B) using EGF monoclonal antibody, clone 3A8 (Cat # MAB14035) (Green). Blue: nuclei. Red: F-actin.
  • ELISA
  • Application Image
  • Immunocytochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1950
  • Gene Name:
  • EGF
  • Gene Alias:
  • HOMG4,URG
  • Gene Description:
  • epidermal growth factor (beta-urogastrone)
  • Gene Summary:
  • Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
  • Other Designations:
  • urogastrone
  • Related Disease
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