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CYLD monoclonal antibody, clone 733

  • Catalog # : MAB14026
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human CYLD.
  • Immunogen:
  • Recombinant protein corresponding to N-terminus of human CYLD.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry (1 ug/mL)
    Immunofluorescence (1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-1:50)
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Jurkat cell lysate with CYLD monoclonal antibody, clone 733 (Cat # MAB14026).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) in the cytoplasm of mouse brain (A) with CYLD monoclonal antibody, clone 733 (Cat # MAB14026) compared to a negative control without primary antibody (B).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of SH-SY5Y cells with CYLD monoclonal antibody, clone 733 (Cat # MAB14026). Panel a: CYLD (Green). Panel b: nuclei (Blue). Panel c: F-actin (Red). Panel d: merge. Panel e: no primary antibody control.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 1540
  • Gene Name:
  • CYLD
  • Gene Alias:
  • CDMT,CYLD1,CYLDI,EAC,FLJ20180,FLJ31664,FLJ78684,HSPC057,KIAA0849,MFT,MFT1,SBS,TEM,USPL2
  • Gene Description:
  • cylindromatosis (turban tumor syndrome)
  • Gene Summary:
  • This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • deubiquitinating enzyme CYLD,ubiquitin carboxyl-terminal hydrolase CYLD,ubiquitin specific peptidase like 2,ubiquitin thiolesterase CYLD,ubiquitin-specific-processing protease CYLD
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