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CTNNB1 monoclonal antibody, clone CAT-5H10

  • Catalog # : MAB14002
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant chicken CTNNB1.
  • Immunogen:
  • Recombinant MBP fusion protein corresponding to a 100 amino acid fragment of the C-terminus of chicken CTNNB1.
  • Host:
  • Mouse
  • Reactivity:
  • Chicken, Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Immunocytochemistry
    Immunofluorescence
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-1:100)
    Immunoprecipitation
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (1% BSA, 0.09% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HeLa cell lysates with CTNNB1 monoclonal antibody, clone CAT-5H10 (Cat # MAB14002).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of mouse stomach tissue (right) with CTNNB1 monoclonal antibody, clone CAT-5H10 (Cat # MAB14002) compared to a negative control without primary antibody (left).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach tissue (right) with CTNNB1 monoclonal antibody, clone CAT-5H10 (Cat # MAB14002) compared to a negative control without primary antibody (left).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma (right) with CTNNB1 monoclonal antibody, clone CAT-5H10 (Cat # MAB14002) compared to a negative control without primary antibody (left).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of 70% confluent log phase HeLa cells with CTNNB1 monoclonal antibody, clone CAT-5H10 (Cat # MAB14002). (Panel a: green) under 1.5 ug/mL working concentration. Nuclei were stained with DAPI (Panel b: blue); F-actin was stained with Alexa Fluor 594 Phalloidin (Panel c: red). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • CTNNB1
  • Gene Alias:
  • CHBCAT
  • Gene Description:
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • Other Designations:
  • beta catenin
  • Interactome
  • Interactome
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