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ATP5B monoclonal antibody, clone 3D5AB1

  • Catalog # : MAB13998
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human ATP5B.
  • Immunogen:
  • Human heart mitochondria.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (3-5 ug x 106 cells)
    Immunocytochemistry (2 ug/mL)
    Immunofluorescence (2 ug/mL)
    Western Blot (0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In HEPES (0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HepG2, Lane 2: SK-OV-3, Lane 3: MDA-MB-231 cell lysates with ATP5B monoclonal antibody, clone 3D5AB1 (Cat # MAB13998).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of 70% confluent log phase HepG2 cells with ATP5B monoclonal antibody, clone 3D5AB1 (Cat # MAB13998) (Panel a: green) under 2 ug/mL working concentration. Nuclei were stained with DAPI (Panel b: blue); F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Panel c: red). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells with ATP5B monoclonal antibody, clone 3D5AB1 (Cat # MAB13998) (red histogram). Pink histogram: mouse isotype control; Purple histogram: unstained control cells; Green histogram: no primary antibody control.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Entrez GeneID:
  • 506
  • Gene Name:
  • ATP5B
  • Gene Alias:
  • Gene Description:
  • ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
  • Gene Summary:
  • This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq
  • Other Designations:
  • ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit,mitochondrial ATP synthetase, beta subunit
  • Related Disease
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