Mouse monoclonal antibody raised against native human APOB.
Purified human serum LDL.
Theoretical MW (kDa):
Ion exchange chromatography purification
ELISA Flow Cytometry (3-5 ug x 106 cells) Immunocytochemistry (2 ug/mL) Immunofluorescence (2 ug/mL) Immunoprecipitation Radioimmunoassay The optimal working dilution should be determined by the end user.
In 0.01 M potassium phosphate, 2.5 mM KCl, 0.135 M NaCl (1 mg/mL BSA, 0.05% sodium azide).
Store at 4°C for up to 6 months. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunofluorescent staining of 70% confluent log phase HepG2 cells with APOB monoclonal antibody, clone F2C4 (Cat # MAB13995). (Panel a: green) under 2 ug/mL working concentration. Nuclei were stained with DAPI (Panel b: blue); F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Panel c: red). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.
Flow cytometric analysis of HepG2 cells with APOB monoclonal antibody, clone F2C4 (Cat # MAB13995) (red histogram). Pink histogram: mouse isotype control; Purple histogram: unstained control cells; Green histogram: no primary antibody control.
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq