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Last updated: 2016/11/27

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APOB monoclonal antibody, clone F2C13

  • Catalog # : MAB13993
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native human APOB.
  • Immunogen:
  • Purified human serum LDL.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 513
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ion exchange chromatography purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA
    Flow Cytometry (3-5 ug x 106 cells)
    Immunocytochemistry (2 ug/mL)
    Immunofluorescence (2 ug/mL)
    Immunoprecipitation
    Radioimmunoassay
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.01 M potassium phosphate, 2.5 mM KCl, 0.135 M NaCl (1 mg/mL BSA, 0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C for up to 6 months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of 70% confluent log phase HepG2 cells with APOB monoclonal antibody, clone F2C13 (Cat # MAB13993). (Panel a: green) under 2 ug/mL working concentration. Nuclei were stained with DAPI (Panel b: blue); F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Panel c: red). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells with APOB monoclonal antibody, clone F2C13 (Cat # MAB13993) (red histogram). Pink histogram: mouse isotype control; Purple histogram: unstained control cells; Green histogram: no primary antibody control.
  • Application Image
  • Immunocytochemistry
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
  • Gene Information
  • Entrez GeneID:
  • 338
  • Gene Name:
  • APOB
  • Gene Alias:
  • FLDB
  • Gene Description:
  • apolipoprotein B (including Ag(x) antigen)
  • Gene Summary:
  • This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000115994,apoB-100,apoB-48,apolipoprotein B,apolipoprotein B48
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