Mouse monoclonal antibody raised against native human APOA1.
Native purified APOA1 from human plasma.
Protein A purification
ELISA (1.0 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200) Immunoprecipitation Radioimmunoassay Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In 0.01 M potassium phosphate, 2.5 mM KCl, 0.135 M NaCl (1 mg/mL BSA, 0.05% sodium azide).
Store at 4°C for up to 6 months. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of HepG2 cell lysates with APOA1 monoclonal antibody, clone 532 (Cat # MAB13991).
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: 250 ng, Lane 2: 125 ng , Lane 3: 62.5 ng recombinant human APOA1 protein and Lane 4: 250 ng, Lane 5: 125 ng , Lane 6: 62.5 ng recombinant mouse APOA1 protein with APOA1 monoclonal antibody, clone 532 (Cat # MAB13991).
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver tissue with APOA1 monoclonal antibody, clone 532 (Cat # MAB13991) (right) compared to a negative control without primary antibody (left).
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq