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Alpha Tubulin monoclonal antibody, clone 236-10501

  • Catalog # : MAB13986
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native bovine alpha-tubulin.
  • Immunogen:
  • Native purified bovine alpha-tubulin.
  • Host:
  • Mouse
  • Reactivity:
  • Bovine, Human, Mouse
  • Form:
  • Lyophilized
  • Purification:
  • Biochemical fractionation
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Flow Cytometry (1:100 - 1:500)
    Immunocytochemistry (1:100 - 1:500)
    Immunofluorescence (1:100 - 1:500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20 - 1:200)
    Western Blot (1:1000 - 1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS.
  • Storage Instruction:
  • Store at -20°C.
    Reconstitute in PBS, containing 1% BSA and 2 mM sodium azide.
    Reconstituted solution should remain stable for 6 months when stored at 4°C. For longer storage, stored the reconstituted solution at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: A-431, Lane 2: Raji, Lane 3: MDA-MB-231, Lane 4: HCT-116, Lane 5: HeLa, Lane 6: DU145, Lane 7: PC-3, and Lane 8: Caco-2 cells with Alpha Tubulin monoclonal antibody, clone 236-10501 (Cat # MAB13986).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human astroglioma tissue (right) with Alpha Tubulin monoclonal antibody, clone 236-10501 (Cat # MAB13986) compared to a negative control without primary antibody (left).
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of NIH/3T3 cell with Alpha Tubulin monoclonal antibody, clone 236-10501 (Cat # MAB13986) and visualized with blue-fluorescent Alexa Fluor® 405 goat anti-mouse IgG antibody.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of bovine pulmonary artery endothelial cell (A) and human HeLa cell (B) with Alpha Tubulin monoclonal antibody, clone 236-10501 (Cat # MAB13986).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cell with Alpha Tubulin monoclonal antibody, clone 236-10501 (Cat # MAB13986) (red histogram). Pink histogram: mouse isotype control; Purple histogram: unstained control cells; Green histogram: no primary antibody control.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
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