Immunofluorescent staining of 70% confluent log phase HepG2 cell with ALPP monoclonal antibody, clone 5B1 (Cat # MAB13978) (Panel a: green) under 2 ug/mL working concentration. Nuclei were stained with DAPI (Panel b: blue); F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Panel c: red). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq